Importance of creating a accurate standard curve

#Importance of creating a accurate standard curve serial

A sample matrix is the components of the sample other than the analyte of interest, including the solvent and all salts, proteins, metal ions, etc. To be completely accurate, the standard samples should be run in the same matrix as the unknown sample. Thus, care must be taken when making the initial solution.Д¬alibration curves can be used to predict the concentration of an unknown sample. The disadvantage is that any errors in solution making-pipetting, massing, etc.-get propagated as more solutions areВ made. The advantage is that only one initial solution is needed. The next sample is made from the previous dilution, and the dilution factor is often kept constant.

#Importance of creating a accurate standard curve serial

With serial dilutions, a concentrated sample is diluted down in a stepwise manner to make lower concentrations. Another method for making many different concentrations of a solution is to use serial dilutions. However, that can take a lot of starting material and be time consuming. When making solutions for a calibration curve, each solution can be made separately. The calibration curve can be used to calculate the limit of detection and limit of quantitation. Typically the response is linear, however, a curve can be made with other functions as long as the function is known. The data are then fit with a function so that unknown concentrations can be predicted. For more accuracy and to understand the error, the response at each concentration can be repeated so an error bar is obtained. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. Jill Venton - University of VirginiaД¬alibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample.